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ISENVO Swine Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Rapid Test Kit Diagnostic Tools for Farm Animal Livestock
|Swine Test Kit
|Taeing+Pit Microchip Reader+Wholesale Manufacturer
|US $ 0.9 / piece
|Terms of Payment
Porcine blue ear disease, also known as Porcine reproductive and respiratory syndrome (PRRS), is an acute, highly infectious viral disease characterized by reproductive disorders and respiratory symptoms caused by Porcine reproductive and respiratory syndrome virus (PRRSV).
The swine blue ear antigen rapid test card is an immunochromatographic immunoassay technique for the qualitative detection of blue ear antigen in swine blood. The sample to be tested is added to the spiked wells and moved along the chromatographic membrane with the colloidal gold-labeled anti-PRRS antibody. If the PRRS antigen is present in the sample, it binds to the antibody on the detection line and shows a burgundy color. If the PRRS antigen is not present in the sample, no color reaction is produced.
1. Blood samples
1) Collect whole blood and isolate the serum (2000-3000 rpm/centrifuge for 5-15 minutes, or overnight natural precipitation at 4°C), or use whole blood with anticoagulant as the test sample. Whole blood without anticoagulant may also be used, but the test must be performed immediately.
2) Tear open the aluminum foil bag, remove the test card and place it on a flat, clean benchtop.
3) Use the matching pipette to draw up the collected sample and slowly and accurately add 2 drops drop by drop to the "S" marked addition hole.
(4) Let the sample stand at room temperature for 10-15 minutes to judge the result, more than 15 minutes results are invalid.
2. Tissue samples
1) Dissect the pig to be examined and find the lesion tissue.
2) Cut off a piece of lesion tissue, add about ten times the volume of saline, and then homogenize the tissue with the sample buffer using a suitable tool.
3) Allow to stand for 2-3 minutes to allow large particles to precipitate.
4) Remove the test card from the sealed bag. Place the test card on a flat surface, pipette the upper clear layer of the homogenate and add 2 drops slowly and accurately drop by drop to the "S" marked spiked well.
5) Let the test stand at room temperature for 10-15 minutes to judge the results, more than 15 minutes results are invalid.