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Cattle&Sheep Chlamydophila Animal Rapid Test Kit For Farm Ranch Breeding Grounds
|Cattle&Sheep Test Kit
|Taeing+Pit Microchip Reader+Wholesale Manufacturer
|US $ 0.9 / piece
|Terms of Payment
The Chlamydiosis antibody gold standard rapid test card for bovine and sheep adopts a rapid immunochromatographic detection technique for the detection of chlamydial antibodies in whole blood (sheep) and serum. After adding the sample to the spiked wells, it moves along the chromatographic membrane together with the colloidal gold-labeled chlamydial antigen. If anti-chlamydial antibodies are present in the sample, they bind to the antigen on the detection line and show a burgundy color. If no chlamydial antibodies are present in the sample, no color reaction is produced.
1. Whole blood is collected and serum is separated (2000-3000 rpm/centrifuge for 5-15 minutes, or overnight at 4°C for natural precipitation).
2. Or use whole blood of sheep with anticoagulant as the test sample. Whole blood of sheep without anticoagulant can also be used, but the test must be performed immediately. Cows can only use serum and not whole blood for testing.
3. Tear open the aluminum foil bag, remove the test card and place it on a flat, clean table.
4. Use the matching pipette to aspirate the collected sample and slowly and accurately add 3 drops drop by drop to the "S" marked addition hole.
5. Place the sample at room temperature for 10-20 minutes to judge the results, more than 20 minutes results are invalid.
1. If the cattle or sheep have not been vaccinated against Chlamydia.
a) When no obvious color band appears at the detection line (T) of the tested sample, it means that there is no Chlamydia antibody in the tested sample. If the cattle and sheep are healthy, then there is no Chlamydia infection.
b) When there is a clear color band at the detection line (T) of the tested sample, the animal has Chlamydia infection or previous infection, which should be further observed and verified.
2. If the cattle or sheep have been vaccinated against Chlamydia.
c) When no obvious color band appears at the detection line (T) of the examined sample, it means that the vaccine immunization has not produced protective antibodies and there is a risk of infection with wild virus, and it is recommended to carry out vaccine replenishment.
d) When there is a clear band at the test line (T) of the sample, it means that the vaccine has produced protective antibodies. However, if the corresponding symptoms of Chlamydia infection appear, it cannot be excluded that such a situation of Chlamydia infection occurred due to immunization failure.
Storage method: 2 - 30ºC.
Expiration date: 24 months.
1. Do not use if the product is found to be expired or the foil bag is damaged.
2. Pure water, tap water, saline cannot be used as negative control. 3.
3. The product can be stored at room temperature in the shade (below 30℃). If stored refrigerated, the test card should be equilibrated to room temperature before opening the aluminum foil pouch to prevent the chromatography membrane from absorbing moisture. The test card should be used as soon as possible after it is removed from the foil pouch.
4. Do not touch the chromatography film at the "sample filling hole" and "viewing window" on the test card.
5. To ensure detection, use fresh, uncontaminated samples as much as possible. If the sample has a large amount of lipids, it will interfere with the test, in this regard, should be centrifuged, avoid the grease layer to take the serum part of the test. When the blood or serum is abnormally viscous or insufficient saline can be used to dilute the sample against times before testing.
6. If the collected serum sample cannot be tested immediately, it should be stored at 4°C for short-term storage (48 hours) and below -20°C for long-term storage.
7. Whole blood specimens without anticoagulant should be tested immediately; if partial coagulation has occurred, please separate the serum for testing. Whole blood specimens with anticoagulant should be tested within 24 hours and should not be frozen; freezing and thawing will cause red blood cells to rupture and hemolysis, which will seriously affect the test results.
8. When adding samples, please keep the front of the pipette at a distance of about 1 cm from the spiking hole to ensure accurate spiking; if the distance is too close, the volume of the droplet may be reduced or the sample volume may be inaccurate due to the error in judging the number of drops, which will affect the test results.
9. When the sample is added to the "spiking hole", foam should be avoided. The sample volume should be limited to about 100ul (about 3 drops of sample). Excessive sample addition will not improve the sensitivity of the test. In particular, an excessive amount of whole blood sample will cause red blood cells to accumulate in the "display well" area, resulting in a deep red background on the chromatography membrane, which will seriously affect the results.
The level of antibodies is only related to the depth of the detection line (T line), but not to the depth of the quality control line (C line). As long as the quality control line is clearly visible, it means the experiment is valid.
11. used test cards and samples must be properly disposed of as contaminants in accordance with the relevant local regulations.